Protocols

We are working together to develop common, best-practice protocols, methods and techniques to sequence SARS-CoV-2 samples

Protocols.io — the canonical source for COG-UK consortium protocols

COG-UK members are working together to develop common, best-practice protocols, methods and techniques to prepare collect and prepare SARS-CoV-2 virus samples for sequencing, conduct short-read and long-read sequencing at scale with minimal turnaround times, and speed the flow of whole-genome data to open-access databases and online visualisation platforms.

All protocols, standards and techniques will be shared between COG-UK members through the protocols.io online platform. All documentation is disseminated here.

 

Current protocols

COVID-19 ARTIC v3 Illumina library construction and sequencing protocol – tailed method

Authors: DNA Pipelines R&D, Benjamin Farr, Diana RajanEmma Betteridge, …, Ian Johnston.
Published: 4 Nov 2020

COVID-19 ARTIC v3 Illumina library construction and sequencing protocol

Authors: DNA Pipelines R&D, Benjamin Farr, Diana RajanEmma Betteridge, …, Ian Johnston.
Published: 4 Nov 2020

nCoV-2019 sequencing protocol v3 (LoCost).

Authors: Josh Quick.
Published: 25 Aug 2020